What is the distance between Fort Myers and Gainesville?

The distance between Fort Myers and Gainesville is around 341 km (212 miles).

What are the departure and arrival locations for buses traveling from Fort Myers to Gainesville?

Buses traveling this route start their trip at Daniels Pkwy & I-75, in Fort Myers, and end it at Rosa Parks Station, in Gainesville.

Bus Fort Myers to Gainesville from $67 | Refundable Tickets

Next bus departures

Next departures for Fort Myers to Gainesville on November 22
Operated by	Vehicle type	Departure time	Departure location	Trip duration	Arrival time	Arrival location	Recommended
	Bus	11:55am	Fort Myers, FL	5h 5m	5:00pm	Gainesville-UF	CheapestFastest
FlixBus + Greyhound Lines, Inc.	Bus	6:30pm	Ft Myers Bus Station	13h 20m	7:50am	Gainesville Bus Stop	No tags
	Bus	2:35pm	Ft Myers Bus Station	10h 30m	1:05am	Rosa Parks Station	No tags
FlixBus + Greyhound Lines, Inc.	Bus	6:30pm	Ft Myers Bus Station	16h 55m	11:25am	Rosa Parks Station	No tags

Next departures for Fort Myers to Gainesville on November 23
Operated by	Vehicle type	Departure time	Departure location	Trip duration	Arrival time	Arrival location	Recommended
	Bus	4:25pm	Ft Myers Bus Station	8h 40m	1:05am	Rosa Parks Station	Cheapest
	Bus	11:55am	Fort Myers, FL	5h 5m	5:00pm	Gainesville-UF	Fastest
FlixBus + Greyhound Lines, Inc.	Bus	6:30pm	Ft Myers Bus Station	13h 20m	7:50am	Gainesville Bus Stop	No tags

Next departures for Fort Myers to Gainesville on November 24
Operated by	Vehicle type	Departure time	Departure location	Trip duration	Arrival time	Arrival location	Recommended
	Bus	11:55am	Fort Myers, FL	5h 5m	5:00pm	Gainesville-UF	CheapestFastest
FlixBus + Greyhound Lines, Inc.	Bus	6:30pm	Ft Myers Bus Station	13h 20m	7:50am	Gainesville Bus Stop	No tags
	Bus	2:35pm	Ft Myers Bus Station	10h 30m	1:05am	Rosa Parks Station	No tags
FlixBus + Greyhound Lines, Inc.	Bus	6:30pm	Ft Myers Bus Station	16h 55m	11:25am	Rosa Parks Station	No tags

Next departures for Fort Myers to Gainesville on November 27
Operated by	Vehicle type	Departure time	Departure location	Trip duration	Arrival time	Arrival location	Recommended
	Bus	11:55am	Fort Myers, FL	5h 5m	5:00pm	Gainesville-UF	CheapestFastest
FlixBus + Greyhound Lines, Inc.	Bus	6:30pm	Ft Myers Bus Station	13h 20m	7:50am	Gainesville Bus Stop	No tags
	Bus	4:25pm	Ft Myers Bus Station	8h 40m	1:05am	Rosa Parks Station	No tags

Last refresh of the prices: Today at 7:59 AM EST.

Get from Fort Myers to Gainesville

This route is served by bus only

The journey takes about 13 hours 20 minutes, and with fares starting at just $72, it presents an excellent value for a comfortable ride.

4bus per day

13h 20mAverage Duration

337 kmDistance

8kgCO₂ emissions

Where are the popular stops in Fort Myers and Gainesville?

Stops in Fort Myers

A
Daniels Pkwy & I-75
13600 Indian Paint Ln (McDonald's on Daniels Pwy and I-75), Fort Myers, FL 33912
B
Colonial Blvd & I-75
Walmart, 4770 Colonial Blvd, Fort Myers, FL 33966
C
Rosa Parks
2250 Widman Way, Fort Myers, FL 33901

Stops in Gainesville

A
Rosa Parks Station
700 SE 3rd St, Gainesville, FL 32601
Visit page
B
Williston Rd & I-75
3509 SW Williston Rd, Gainesville, FL 32608, USA
Visit page
C
Greyhound Station
101 NE 23rd Ave, Gainesville, FL 32609
Visit page

Compare bus companies

FlixBus

3.5/511,393 reviews

Staff4.2

Timeliness3.9

Cleanliness4.1

Wifi2.7

RedCoach

4/54,065 reviews

Staff4.6

Timeliness3.9

Cleanliness4.6

Wifi3.8

Based on 4065 reviews, the company was rated 4 stars on Busbud. Travellers were especially satisfied with the ticket access and the staff but often complained with the WiFi. RedCoach ticket prices on this trip start at $77

RedCoach Fort Myers Gainesville recent customer reviews

Well my bus was 30 minutes late. When it got there a 9:00, it let people off while I waited to get on and then it left at 9:03 leaving me there. I was running behind it waving and it never stopped. So I didn’t ever make it to my stop. I got there early, waited past time it should have been there just to have it leave me. I called customer service and they were no help at all, telling me all they could do was get me on the next bus which was no help because I had to be in Gainesville at 3, the next bus left at 2. I asked if they could give me a credit for another time when I could use the bus and the lady said it wasn’t possible.

Courtney R.

November 14, 2019

Bus was very comfortable and on time. Driver could of been a little friendlier, but definitely will be riding again.

Kevin G.

October 3, 2019

It was a great comfortable trip

Adrienne H.

December 20, 2019

US Bus Companies: FlixBus, BlaBlaCar Bus, ALSA, Greyhound, Intercape, Trenitalia, Sindbad

Frequently asked questions about travelling from Fort Myers to Gainesville, FL by bus

What is the distance between Fort Myers and Gainesville?
The distance between Fort Myers and Gainesville is around 341 km (212 miles).
What are the departure and arrival locations for buses traveling from Fort Myers to Gainesville?
Buses traveling this route start their trip at Daniels Pkwy & I-75, in Fort Myers, and end it at Rosa Parks Station, in Gainesville.

Find cheapest prices for buses

Price is for a one-way ticket for one passenger and based on past searches.

Best price found

11-19

$97

11-20

$96

Today

$115

11-22

$106

11-23

$115

11-24

$106

11-25

No data

11-26

$110

The cheapest trip from Fort Myers to Gainesville, FL was searched and found on Nov 20, 2025 with a price of $96

To save money and be sure you have the best seat, it's a good idea to buy your bus tickets from Fort Myers to Gainesville, FL, as early as possible.

You can expect to pay from $96 to $132 for a bus ticket from Fort Myers to Gainesville, FL based on the last 2 days. You can expect to find the cheapest price for the trip at $106 which is on 2025-11-22.

Usually FlixBus will charge you higher prices for tickets closer to the departure date. To find the best prices, you should be flexible on your date of travel.